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| Registros recuperados: 16 | |
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| Soletchnik, Patrick; Le Moine, Olivier; Faury, Nicole; Guilpain, Patrice; Geairon, Philippe; Razet, Daniel; Seugnet, Jean-luc; Robert, Stephane; Ropert, Michel; Simonne, Charlotte; Hugonnet, Virginie; Moal, Jeanne; Pouvreau, Stephane; Huvet, Arnaud; Samain, Jean-francois; Lambert, Christophe; Soudant, Philippe; Frouel, Stephane; Choquet, Gwenaelle; Delaporte, Maryse; Paillard, Christine. |
| Four laboratories are involved in this multidisciplinary study: the Conchological Laboratory of Normandie (LCN), the Invertebrates Physiology Laboratory (LPI), the Université Bretagne Occidentale (LEMAR IHP) and the Conchological Laboratory of Poitou-Charentes (LCPC). The part of the study which is presented here (thematics "Coastal ecology") was produced in collaboration with the LCN. The data on the Bay of Veys was collected by Michel Ropert. This study aims at observing the "sites" effect on mortality and on the general biology of the animal, as well as measuring some environmental elements (e.g. water temperature, etc.). The geographical sites are Gefosse in the Bay of Veys (East Cotentin) and the Ronce bank in the south of the Marennes Oléron Bay. The... |
| Tipo: Text |
Palavras-chave: Immunologie; Physiologie; Paramètres environnementaux; Croissance; Ecologie côtière; Baies des Veys Cotentin; Ronce Charente Maritime; Comparaison sites; Reproduction; Mortalités; Crassostrea gigas; Huîtres. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/acte-3405.pdf |
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| Delaporte, Maryse; Chu, Fu-lin; Langdon, Chris; Moal, Jeanne; Lambert, Christophe; Samain, Jean-francois; Soudant, Philippe. |
| The aim of this study was to assess the effect of dietary eicosapentaenoic acid (20:5n-3) on hemocyte parameters such as hemocyte concentration, phagocytosis, and non-stimulated reactive oxygen species (ROS) production in Pacific oysters Crassostrea gigas, as well on proximate biochemical and fatty acid compositions. One-year-old oysters (C. gigas) were fed T-Isochrysis aff. galbana(T-Iso), which is low in 20:5n-3, either alone or with supplements of a lipid emulsion rich in 20:5n-3 at 1%, 10% or 50% (dry weight of the algal ration) for up to 7 weeks. Changes in gill fatty acid composition demonstrated that the lipid emulsion was well ingested by oysters during the dietary conditioning. Biochemical analysis indicated that oysters fed supplements of 50%... |
| Tipo: Text |
Palavras-chave: Polyunsaturated fatty acids; Immunology; Hemocytes; Eicosapentaenoic acid 20 : 5n 3; Crassostrea gigas; Bivalves. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2604.pdf |
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| Delaporte, Maryse; Soudant, P; Lambert, C; Jegaden, M; Moal, Jeanne; Pouvreau, Stephane; Degremont, Lionel; Boudry, Pierre; Samain, Jean-francois. |
| Within the framework of a national scientific program named "MORtalites ESTivales de l'huitre creuse Crassostrea gigas" (MOREST), a family-based experiment was developed to study the genetic basis of resistance to summer mortality in the Pacific oyster, Crassostrea gigas. As part of the MOREST project, the second generation of three resistant families and two susceptible families were chosen and pooled into two respective groups: "R" and "S". These two groups of oysters were conditioned for 6 months on two food levels (4% and 12% of oyster soft-tissue dry weight in algal dry weight per day) with a temperature gradient that mimicked the Marennes-Oleron natural cycle during the oyster reproductive period. Oyster mortality remained low for the first two... |
| Tipo: Text |
Palavras-chave: Summer mortality; Reproduction; Reactive oxygen species ROS; Hemocyte parameters; Genetic selection; Crassostrea gigas. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3578.pdf |
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| Delaporte, Maryse; Soudant, Philippe; Moal, Jeanne; Lambert, Christophe; Quere, Claudie; Miner, Philippe; Choquet, Gwenaelle; Paillard, Christine; Samain, Jean-francois. |
| The impact of diets upon the fatty acid composition of haemocyte polar lipids and consequently upon immune parameters has been tested in the oyster Crassostrea gigas and the clam Ruditapes philippinarum. Oysters and clams were fed each of three cultured algae: Chaetoceros calcitrans, which is rich in 20:5(n-3) and 20:4(n-6) and poor in 22:6(n-3) fatty acids; T-Iso (Isochrysis sp.), which is rich in 22:6(n-3) and deficient in 20:5(n-3) and 20:4(n-6); and Tetraselmis suecica, which is deficient in 22:6(n-3) and contains only small amounts of 20:5(n-3) and 20:4(n-6). Fatty acid composition of haemocyte polar lipids was greatly affected by the diet. Oysters and clams fed C. calcitrans maintained a higher proportion of 20:5(n-3) and 20:4(n-6) in their haemocyte... |
| Tipo: Text |
Palavras-chave: Ruditapes philippinarum; Crassostrea gigas; Polyunsaturated fatty acids; Phagocytosis; Oxidative activity; Haemocyte membrane; Nutrition; Immunology; Bivalve; Immunologie; Bivalve; Ruditapes philippinarum; Crassostrea gigas; Acides gras poly insatures; Phagocytose; Flambee respiratoire; Membrane; Hemocyte; Nutrition. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-354.pdf |
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| Delaporte, Maryse; Moal, Jeanne; Soudant, Philippe; Lambert, Christophe; Pouvreau, Stephane; Enriquez, Martha; Degremont, Lionel; Soletchnik, Patrick; Gagnaire, Beatrice; Ropert, Michel. |
| In the frame of MOREST project, a common biological material, resulting of a mixture of different families produced in experimental hatchery, was reared in two different environmental fields : Normandy and Charente. Concomitantly, a pool was conditioned at the Ifremer Argenton hatchery with three different algae levels : 4%, 8% and 12% of algal dry weight per oyster dry weight. During the experiments, five immune parameters were studied in parallel with survival rate and reproductive status (stages and intensity). Site location, seasonal variations and experimental diet level clearly influenced oyster immune parameters. Haemocyte counts were higher for oysters reared in Normandy than those reared in Charente and in hatchery. Granulocyte percentage was... |
| Tipo: Text |
Palavras-chave: Defense mechanisms; Nutrition; Seasonal variations; Physiology; Crassostrea gigas; Pacific oysters. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/acte-3500.pdf |
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| Delaporte, Maryse. |
| Des études ont déjà montré que la composition lipidique des micro-algues utilisées dans les écloseries et nurseries avait des répercussions sur la croissance, le développement et la survie des bivalves. L'objectif de la présente étude est de déterminer l'influence de l'alimentation sur la composition membranaire des hémocytes et sur les mécanismes de défense des bivalves. Des huîtres, Crassostrea gigas, et des palourdes, Ruditapes philippinarum, ont été nourries avec trois algues: Chaetoceros calcitrans déficiente en 22:6(n-3), lsochrysis galballa déficiente en 20:5(n-3) et 20:4(n-6), Tetraselmis suecica déficiente en 22:6(n-3), 20:5(n- 3) et 20:4(n-6). Dans notre étude, les animaux nourris avec T. suecica présentent le plus faible indice de condition. Les... |
| Tipo: Text |
Palavras-chave: Acide gras; Immunologie; Hémocytes; Composition membranaire; Phagoyctyose; Activité métabolique. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/00439/55108/56560.pdf |
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| Samain, Jean-francois; Degremont, Lionel; Soletchnik, Patrick; Haure, Joel; Bedier, Edouard; Ropert, Michel; Moal, Jeanne; Huvet, Arnaud; Bacca, Helene; Van Wormhoudt, A; Delaporte, Maryse; Costil, K; Pouvreau, Stephane; Lambert, Christian; Boulo, Viviane; Soudant, Philippe; Nicolas, Jean-louis; Leroux, Frederique; Renault, Tristan; Gagnaire, Beatrice; Geret, Florence; Boutet, Isabelle; Burgeot, Thierry; Boudry, Pierre. |
| Summer mortality of Pacific oysters is known in several countries. However no specific pathogen has been systematically associated with this phenomenon. A complex combination of environmental and biological parameters has been suggested as the cause and is now starting to be identified. A high genetic basis was found for survival in oysters when a first generation (G1) was tested in three sites during summer. This paper presents a synthesis on physiological characteristics of two selected groups ('R' and 'S', from families selected for resistance and susceptibility to summer mortality respectively), of the second and third generations. R and S showed improvement or reduction of survival compared with the control in both field and laboratory trials... |
| Tipo: Text |
Palavras-chave: ROS; Temperature; Stress; Hemocytes; Reproduction; Genetic selection; Resistant oyster; Crassostrea gigas; Pacific Oyster; Summer mortality. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2809.pdf |
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| Lambert, Christophe; Soudant, Phillippe; Degremont, Lionel; Delaporte, Maryse; Moal, Jeanne; Boudry, Pierre; Jean, Frédéric; Huvet, Arnaud; Samain, Jean-francois. |
| High variability among individuals is often encountered when hemocyte characteristics are measured in bivalves. Such variability is suspected to result partly from genetic factors. In this study, hemocyte characteristics of six families of Crassostrea gigas were compared by flow cytometry at one sampling date in October 2001. These families were obtained from a nested, half-sibling cross design, and reared from July to October 2001 at three sites distributed along the French Atlantic coast from north to south: Baie des Veys (Normandy), Riviere d'Auray (Brittany) and Ronce (Marennes-oleron Basin, Poitou Charentes). Among the 15 measured hemocyte characteristics, production of reactive oxygen species (ROS) of untreated hemocytes (maintained in filtered... |
| Tipo: Text |
Palavras-chave: Genetic; Flow cytometry; Reactive oxygen species ROS; Adhesion; Hemocyte; Crassostrea gigas; Summer mortality. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3054.pdf |
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| Delaporte, Maryse; Soudant, Philippe; Moal, Jeanne; Giudicelli, Emilie; Lambert, Christophe; Seguineau, Catherine; Samain, Jean-francois. |
| Arachidonic acid (20:4n-6, ArA) and its eicosanoid metabolites have been demonstrated to be implicated in immune functions of vertebrates, fish, and insects. Thus, the aim of this study was to assess the impact of ArA supplementation on the FA composition and hemocyte parameters of oysters Crassostrea gigas. Oyster dietary conditioning consisted of direct addition of ArA solutions at a dose of 0, 0.25, or 0.41 mu g ArA per mL of seawater into tanks in the presence or absence of T-Iso algae. Results showed significant incorporation of ArA into gill polar lipids when administered with algae (up to 19.7%) or without algae (up to 12.1%). ArA supplementation led to an increase in hemocyte numbers, phagocytosis, and production of reactive oxygen species by... |
| Tipo: Text |
Palavras-chave: Hemocyte parameters; Oyster immunity; Arachidonic Acid; Poly unsaturated fatty acids; Nutrition; Bivalve; Crassostrea gigas. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1876.pdf |
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| Delaporte, Maryse; Soudant, P; Lambert, C; Moal, Jeanne; Pouvreau, Stephane; Samain, Jean-francois. |
| The aim of this study was to test the effect of food quantity on energy storage and defense capacities of oysters during a reproductive cycle. One-year-old Crassostrea gigas oysters were fed two different dietary rations (4% and 12% of oyster dry weight in algal dry weight per day) in controlled experimental conditions over an annual cycle. Oyster dry weights, carbohydrate and lipid contents, energetic adenylate charge, and hemocyte parameters of oysters were significantly affected by reproductive processes related to seasonal temperature variation and, to a lesser extent, by the dietary rations. Energy parameters decreased during gametogenesis as gonads developed then increased during the gonad resorption phase. The additional energy provided to oysters... |
| Tipo: Text |
Palavras-chave: Reactive oxygen species production; Reproduction; Phagocytosis; Hemocyte parameters; Energy storage; Crassostrea gigas. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1549.pdf |
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| Lambert, Christophe; Soudant, Philippe; Jegaden, Marine; Delaporte, Maryse; Labreuche, Yannick; Moal, Jeanne; Samain, Jean-francois. |
| Bivalve hemocyte competence has been measured by quantifying functional characteristics, including reactive oxygen intermediate (ROI) production after activation with zymosan or phorbol myristate acetate (PMA). However, untreated oyster hemocytes also produce ROI and RNI (reactive nitrogen intermediates) after bleeding even if not stimulated by Zymosan or PMA. Extensive investigation of this parameter by flow cytometry showed that, in vitro, ROI/RNI production by untreated hemocytes maintained in seawater appeared to be independent of both bacterial burden in the serum and non-self particle phagocytosis. ROI/ RNI production in granulocytes was higher than in hyalinocytes and could be intensified when activated by zymosan but not by PMA. Both cell types... |
| Tipo: Text |
Palavras-chave: NO synthase; NADPH oxidase; Flow cytometry; Reactive nitrogen intermediate RNI; Reactive oxygen intermediate ROI; Hemocytes; Crassostrea gigas. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3057.pdf |
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| Bacca, Helene; Huvet, Arnaud; Fabioux, Caroline; Daniel, Jean-yves; Delaporte, Maryse; Pouvreau, Stephane; Van Wormhoudt, A; Moal, Jeanne. |
| To investigate the control at the mRNA level of glycogen metabolism in the cupped oyster Crassostrea gigas, we report in the present paper the cloning and characterization of glycogen phosphorylase and synthase cDNAs (Cg-GPH and Cg-GYS, respectively, transcripts of main enzymes for glycogen use and storage), and their first expression profiles depending on oyster tissues and seasons. A strong expression of both genes was observed in the labial palps and the gonad in accordance with specific cells located in both tissues and ability to store glucose. Cg-GPH expression was also found mainly in muscle suggesting ability to use glycogen as readily available glucose to. supply its activity. For seasonal examinations, expression of Cg-GYS and Cg-GPH genes... |
| Tipo: Text |
Palavras-chave: Reproduction; Regulation; Oyster; Glycogen; Expression; Gene; Energy; Crassostrea gigas; Bivalve. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-435.pdf |
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| Seguineau, Catherine; Soudant, P; Moal, Jeanne; Delaporte, Maryse; Miner, Philippe; Quere, Claudie; Samain, Jean-francois. |
| The present study tested two techniques for dietary supplementation of Crassostrea gigas spat with PUFA, such as arachidonic acid (AA). The first technique consisted of a preliminary enrichment and growth of an algal concentrate (T-ISO, Isochrysis sp.) with AA dissolved in an ethanol solution, the whole culture then being fed to the spat. This enrichment increased the AA weight percentage in T-ISO neutral and polar lipids from 0.6 to 22.4% and from 0.4 to 6.8%, respectively. The second delivery technique was direct addition separately of free AA dissolved in ethanol solution and algal concentrate (T-ISO + AA) to the spat-rearing tank. To test the efficiency of these delivery techniques, oyster spat were supplemented with AA-enriched T-ISO, T-ISO + AA, and... |
| Tipo: Text |
Palavras-chave: Food supply; Arachidonic acid; Spat; Crassostrea gigas; Dietary. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-1094.pdf |
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| Seguineau, Catherine; Racotta, Ilie; Palacios, Elena; Delaporte, Maryse; Moal, Jeanne; Soudant, Philippe. |
| In a previous study, dietary supplementation with arachidonic acid (ARA) to oysters Crassostrea gigas increased haemocyte numbers, phagocytosis, and production of reactive oxygen species level (ROS) by haemocytes (Delaporte et al., 2006). To assess if the observed stimulation of these cellular responses resulted from changes of ARA-related prostaglandin (PG) production, we analysed prostaglandin E2 metabolite (PGEM) content on the same oysters fed three levels of ARA. Dietary supply of polyunsaturated fatty acids (PUFA) could also induce an oxidative stress that could similarly increase cellular responses; therefore, two indicators of oxidative stress were analysed: peroxidation level and antioxidant defence status. Together the observed positive... |
| Tipo: Text |
Palavras-chave: Prostaglandins; N-6 unsaturated fatty acids; Lipid peroxidation; Antioxidants; ROS production. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00040/15103/12785.pdf |
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| Registros recuperados: 16 | |
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